Tea polyphenols reduces the apoptosis of spermatogenic cells in rats with experimental varicocele.
- Author:
Zheng-hua WU
;
Xin-wen KE
;
Shao-yong FENG
;
Li ZHANG
;
Jin-feng WU
;
Wei CHENG
;
Jian-jun CHENG
;
Jian-dong ZHANG
;
Yan-gang ZHANG
- Publication Type:Journal Article
- MeSH: Animals; Apoptosis; drug effects; Caspase 3; Cytochromes c; metabolism; Dose-Response Relationship, Drug; Hypoxia-Inducible Factor 1, alpha Subunit; metabolism; Ligation; Male; Polyphenols; administration & dosage; pharmacology; Proto-Oncogene Proteins c-bcl-2; metabolism; Random Allocation; Rats; Rats, Wistar; Renal Veins; Spermatozoa; drug effects; Tea; chemistry; Testis; metabolism; Varicocele; complications; metabolism; bcl-2-Associated X Protein; metabolism
- From: National Journal of Andrology 2015;21(8):702-707
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo study the effect of tea polyphenols (TP) on the apoptosis of germ cells in rats with experimental varicocele.
METHODSThirty-two adolescent male Wistar rats were randomly and equally divided into groups A (sham-operation), B (high-dose TP), C (low-dose TP), and D (experimental left varicocele). Experimental varicocele was induced by partial ligation of the left renal vein in the latter three groups of rats. The animals in groups A and D were fed with normal saline, while those in B and C with TP at 40 and 10 mg per kg per d, respectively, all for 4 weeks. Then, all the rats were sacrificed and the left testes harvested for determination of the expression of HIF-1, Bcl-2, Bax, CytC, and caspase-3 by immunohistochemistry and measurement of the apoptosis index (AI) of spermatogenic cells.
RESULTSThe expression of Bcl-2 was higher in groups B and C than in D but lower than in A (P < 0.05), and lower in C than in B (P < 0.05). However, the expressions of HIF-1, Bax, CytC, and caspase-3 were lower in groups B and C than in D but higher than in A (P < 0.05), and higher in C than in B (P < 0.05). The AI of spermatogenic cells was the lowest in group A, higher in D than in the other groups but lower in B than in C (P < 0.05).
CONCLUSIONTP can reduce the apoptosis of spermatogenic cells in a dose-dependent manner in varicocele rats.