Effect of 0.1 Gy X-ray irradiation on gene expression profiles in normal human lymphoblastoid cells.
- Author:
Jie-qing LI
1
;
Dian-jun HOU
;
Ya MA
;
Li FENG
;
Wei-guo LI
;
Wei LIU
;
Jian-wei QIAO
;
Xi-ming JIA
;
Kun LI
Author Information
- Publication Type:Journal Article
- MeSH: Cell Line; Humans; Lymphocytes; radiation effects; Oligonucleotide Array Sequence Analysis; Radiation Dosage; Radiation, Ionizing; Transcriptome; X-Rays
- From: Chinese Journal of Industrial Hygiene and Occupational Diseases 2013;31(10):749-752
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo investigate the effect of 0.1 Gy X-ray irradiation on the gene expression profiles in normal human lymphoblastoid cells using gene microarray and to explore the possible mechanism of the biological effect of low-dose irradiation.
METHODSThe NimbleGen 12×135 K microarray corresponding to 45033 genes was used to analyze the gene expression profiles in AHH-1 cells cultured for 6 h and 20 h after 0.1 Gy X-ray irradiation. A gene was identified as the differentially expressed gene if the ratio between its expression levels in irradiation group and control group was higher than 2 or lower than 0.5. RT-PCR and real-time PCR were used to confirm some differentially expressed genes.
RESULTSThere were 760 up-regulated genes and 1222 down-regulated genes in the cells at 6 h after 0.1 Gy X-ray irradiation, while there were 463 up-regulated genes and 753 down-regulated genes at 20 h after 0.1 Gy X-ray irradiation; there were 92 differentially expressed genes in common. The expression of GADD45A, CDKN2A, and Cx43 measured using gene microarray was confirmed by RT-PCR and real-time PCR.
CONCLUSIONLow-dose irradiation can affect the expression of many functional genes, which provides a basis for the research on the mechanism of radiation damage.