Study on sperm damage caused by trichloroethylene in male rats.
- Author:
De-sheng WU
1
;
Lin-qing YANG
1
;
Sui HUANG
1
;
Jian-jun LIU
1
;
Xin-yun XU
1
;
Hai-yan HUANG
1
;
Chun-mei GONG
1
;
Gong-hua HU
1
;
Qing-cheng LIU
1
;
Xi-fei YANG
1
;
Wen-xu HONG
1
;
Li ZHOU
1
;
Xin-feng HUANG
1
;
Jian-hui YUAN
1
;
Zhi-xiong ZHUANG
2
Author Information
- Publication Type:Journal Article
- MeSH: Animals; Apoptosis; drug effects; Male; Membrane Potential, Mitochondrial; drug effects; Rats; Rats, Sprague-Dawley; Sperm Motility; drug effects; Spermatozoa; cytology; drug effects; Trichloroethylene; toxicity
- From: Chinese Journal of Industrial Hygiene and Occupational Diseases 2013;31(11):811-814
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo study in vitro sperm damage caused by trichloroethylene in male rats.
METHODSSperms of Sprague-Dawley (SD) rats were collected 4 hours after being contaminated by trichloroethylene of 0, 2, 4, 6, 8, and 10 mmol/L in vitro. Giemsa staining was performed to observe the morphological changes of sperms, and flow cytometer was used to detect the changes in mitochondrial membrane potential.
RESULTSThe sperm motilities in 6, 8, and 10 mmol/L trichloroethylene groups decreased significantly compared with that in control group (P <0.01); the sperm aberration rates in 8 and 10 mmol/L trichloroethylene groups were significantly higher than that in control group (P<0.01). With the increase in exposure dose, the proportion of sperms with reduced mitochondrial membrane potential increased, and there were significant differences in sperm apoptosis rate between the 4, 6, 8, and 10 mmol/L trichloroethylene groups and control group (P<0.01).
CONCLUSIONIn vitro exposure to trichloroethylene can reduce sperm motility and increase the aberration rate and apoptosis rate of sperms in male SD rats.
