Proteomic Analysis of the Uterosacral Ligament in Postmenopausal Women with and without Pelvic Organ Prolapse.
- Author:
Zhi-Jing SUN
;
Lan ZHU
1
;
Jing-He LANG
;
Zhao WANG
;
Shuo LIANG
Author Information
- Publication Type:Journal Article
- MeSH: Actins; metabolism; Aged; Apolipoprotein A-I; metabolism; Cyclophilin A; metabolism; Cytoskeleton; metabolism; Female; Flavoproteins; metabolism; Galectin 1; metabolism; Humans; Ligaments; metabolism; Microfilament Proteins; metabolism; Middle Aged; Muscle Proteins; metabolism; Myosins; metabolism; Pelvic Organ Prolapse; metabolism; Postmenopause; metabolism; Proteomics; methods; Reverse Transcriptase Polymerase Chain Reaction; Sacrum; metabolism; Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization; Uterus; metabolism
- From: Chinese Medical Journal 2015;128(23):3191-3196
- CountryChina
- Language:English
-
Abstract:
BACKGROUNDPelvic organ prolapse (POP) is a major health problem in adult women that involves many factors. No proteomic analysis has been conducted exclusively in POP patients. This study aimed to identify the differential expression of proteins that may be involved in POP by proteomic analysis.
METHODSSamples of the uterosacral ligament (USL) were collected from five POP patients and five non-POP patients matched according to age, parity, and menopausal status and analyzed using two-dimensional electrophoresis and matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF-MS). Quantitative real-time polymerase chain reaction (qRT-PCR) was used to verify the mRNA expression of proteins that showed differential expression in the proteomic analyses.
RESULTSProteins differentially expressed between POP and non-POP patients were detected. Eight proteins that were down-regulated in the POP group were identified by MALDI-TOF-MS. These proteins included electron transfer flavoprotein, apolipoprotein A-I, actin, transgelin, cofilin-1, cyclophilin A, myosin, and galectin-1, and their expression was verified by qRT-PCR.
CONCLUSIONUsing comparative proteomics, we identified eight differentially expressed proteins (including four cytoskeleton proteins and three proteins related to apoptosis) in the USL that may be involved in apoptosis associated with the tissue effects in POP pathophysiology.