Bioinformatics analysis of geranylgeranyl pyrophosphate synthase coding gene and amino acid sequence in Lamiaceae.
10.19540/j.cnki.cjcmm.20170103.030
- Author:
Yi-Jun CHEN
1
;
Qi-Xian RONG
2
;
Dan JIANG
2
;
Ye SHEN
2
;
Lu-Qi HUANG
2
Author Information
1. Jiangxi University of Traditional Chinese Medicine, Nanchang 330004, China.
2. State Key Laboratory Breeding Base of Dao-di Herbs, National Resource Center for Chinese Materia Medica, China Academy of Chinese Medical Sciences, Beijng 100700, China.
- Publication Type:Journal Article
- Keywords:
Lamiaceae;
bioinformatics;
diterpene biosynthesis;
geranylgeranyl pyrophosphate synthase (GGPS)
- From:
China Journal of Chinese Materia Medica
2017;42(3):465-472
- CountryChina
- Language:Chinese
-
Abstract:
Geranylgeranyl pyrophosphate synthase enzyme is one of the key enzymes in the synthesis pathway of diterpenoid. Nine Lamiaceae genus GGPS synthase in Genebank was analyzed in this article. GGPS synthase the nucleic acid sequences and amino acid sequences, physicochemical properties, the signal peptide, leader peptides, transmembrane topological structure, hydrophobic, hydrophilic, subcellular localization, secondary structure, function domain, tertiary structure and evolutional relationship were predicted by using bioinformatics methods.Phylogenetic tree was constructed for the geranylgeranyl pyrophosphate synthase enzyme protein family. The results showed that GGPS amino acid sequence of the physical and chemical properties were basically identical, mainly hydrophilic protein, there existed chloroplast transit peptide, and no signal peptide and membrane structure domain, which mainly located in the chloroplast, the minor part located in mitochondria. The main secondary structures of the proteins are alpha helix and random coil. All these proteins have catalytic residues, aspartate-rich region, active site lid residues, substrate-Mg2+ binding site. The results provide theoretical reference for study on both the enzymatic characteristics of GGPS and the biosynthesis pathway of diterpenoid.
