Rapid characterization of chemical constituents in capillary wormwood extract based on UHPLC-LTQ-Orbitrap.
10.19540/j.cnki.cjcmm.2017.0010
- Author:
Wen-Zhu OUYANG
1
;
Zhan-Peng SHANG
2
;
Wen-Jian WANG
1
;
Shou-Xu WANG
3
;
Jia-Xu SHEN
4
;
Hao QIAN
5
;
Zhi-Guo MA
5
;
Jia-Yu ZHANG
2
Author Information
1. Department of Biological and Chemical Engineering, Bozhou University, Bozhou 236800, China.
2. Beijing Research Institution of Chinese Medicine, Beijing University of Chinese Medicine, Beijing 100029, China.
3. Huatuo Medicines Company Limited, Bozhou 236800, China.
4. BozhouHuqiao Medicines Company Limited, Bozhou 236800, China.
5. College of Pharmacy, Jinan University, Guangzhou 510632, China.
- Publication Type:Journal Article
- Keywords:
UHPLC-LTQ-Orbitrap MS;
capillary wormwood extract;
chemical constituent identification
- From:
China Journal of Chinese Materia Medica
2017;42(3):523-530
- CountryChina
- Language:Chinese
-
Abstract:
A rapid and sensitive UHPLC-HR-MSn method was developed for the identification of chemical constituents in capillary wormwood extract. ACQUITY UHPLC HSS T3 chromatography column (2.1 mm×100 mm, 1.7 μm) was used with 0.1% formic acid-acetonitrile solution as the mobile phase in gradient elution. The extract was detected by ESI-LTQ-Orbitrap equipped with an ESI ion source in a negative mode. Based on the accurate mass measurements, retention time, mass fragmentation patterns and literature reports, a total of 50 compounds including 21 flavonoids, 22 phenolic acids, 6 coumarins and 1 other compound were tentatively screened and characterized. These results are helpful for the comprehensive quality control, better comprehension of the metabolism and further study of pharmacodynamic substance from capillary wormwood extract.
