Effect of clinical doses of Realgar-Indigo Naturalis formula and large-dose of realgar on CYP450s of rat liver.
10.19540/j.cnki.cjcmm.20170103.019
- Author:
Huan-Hua XU
1
;
Mei-Xi WANG
1
;
Hong-Ling TAN
2
;
Yu-Guang WANG
2
;
Xiang-Lin TANG
2
;
Cheng-Rong XIAO
2
;
Hua LI
3
;
Yue GAO
1
;
Zeng-Chun MA
1
Author Information
1. Guangdong Pharmaceutical University, Guangzhou 510006, China.
2. Institute of Radiation Medicine, Academy of Military Medical Sciences, Beijing 100850, China.
3. Institute of Pharmacology and Toxicology, Academy of Military Medical Sciences, Beijing 100850, China.
- Publication Type:Journal Article
- Keywords:
Realgar-Indigo Naturalis formula;
cytochrome P450;
enzyme activity;
liver microsome;
realgar
- From:
China Journal of Chinese Materia Medica
2017;42(3):593-599
- CountryChina
- Language:Chinese
-
Abstract:
To investigate the effect of clinical dose of Realgar-Indigo Naturais formula (RIF) and large-dose of Realgar on main drug-metabolizing enzymes CYP450s of rat liver, as well as its regulatory effect on mRNA expression. Wistar rats were administrated orally with tested drugs for 14 days. A Cocktail method combined with HPLC-MS/MS was used in the determination of 4 cytochrome P450 isozymes (CYP1A2, CYP2B, CYP3A and CYP2C) in liver of the rats, and the mRNA expression levels of the above subtypes were detected by real-time fluorescent quantitative PCR. The results showed that RIF can significantly induce CYP1A2 and CYP2B enzyme activity, and inhibit CYP3A enzyme activity. This result was consistent with the mRNA expression. However, its single compound showed weaker or even contrary phenomenon. Different doses of Realgar also showed significant inconsistencies on CYP450 enzymes activity and mRNA expression. These phenomena may be relevant with RIF compatibility synergies or toxicity reduction. The results can also prompt drug interactions when RIF is combined with other medicines in application.
