Optimize ethanol-extraction procedure of Shenlian granules by orthogonal design with pharmacodynamics index.

Author: Min WANG ¹ ; Shou-Ying DU ¹ ; Xiao-Nan CHEN ¹ ; Zhi-Hao TIAN ¹ ; Yang LU ¹ ; Jie BAI ¹ ; Hui-Chao WU ¹ ; Qin-Shuai ZHANG ¹ ; Jie WAN ²
Author Information

1. Beijing University of Chinese Medicine, Beijing 100102, China.
2. Dongfang Hospital, Beijing University of Chinese Medicine, Beijing 100078, China.
Publication Type:Journal Article
Keywords: Shenlian formulaion; ethanol-extraction; multi-index comprehensive weighted score evaluation; orthogonal test
From: China Journal of Chinese Materia Medica 2017;42(4):702-707
CountryChina
Language:Chinese
Abstract: To optimize the ethanol extraction process for Shenlian formula. On the basis of the pharmacodynamics index for different extraction process routes, the contents of salvianolic acid B, tanshinone ⅡA and berberine, as well as the extraction ratio in different experimental schemes were used as the ethanol extraction examining indexes, and multi-criterion synthesizing grading method was used for data analysis to optimize and verify the ethanol-extraction process conditions in orthogonal experiment. The optimum ethanol extraction process was as follows: adding 60% ethanol, 10 times amount, extracting for 2.0 h each time for a total of 2 times. This extraction process showed good stability and availability.