Establish quality evaluation method based on standard decoction of Danshen extract.
10.19540/j.cnki.cjcmm.20170223.011
- Author:
Qing DONG
1
;
Hua-Tao YU
2
;
Yun-Tao DAI
2
;
Jung CHAO
2
;
Zi-Quan FAN
3
;
Dan-Dan WANG
3
;
Chao ZHU
4
;
Shi-Lin CHEN
2
Author Information
1. Institute of Chinese Materia Medica, China Academy of Chinese Medical Sciences, Beijing 100700, China.
2. Institute of Chinese Materia Medica, China Academy of Chinese Medicinal Sciences, Beijing 100700, China.
3. Waters Corporation(Shanghai), Shanghai 201206, China.
4. College of Medicine and Nursing, Dezhou University, Dezhou 253023, China.
- Publication Type:Journal Article
- Keywords:
Salviae Miltiorrhizae Radix et Rhizoma;
extraction ratio;
quality evaluation;
standard decoction;
transfer rate
- From:
China Journal of Chinese Materia Medica
2017;42(5):817-822
- CountryChina
- Language:Chinese
-
Abstract:
The quality of Danshen extract granules on market is largely different from each other mainly due to the heterogeneous quality of raw materials of Salvia miltiorrhiza, various producing procedures and lack of good quality evaluation method. Formula granule and "standard decoction" have the same quality. In this paper, a systematic evaluation method for the quality of Danshen decoction was established from the perspective of "standard decoction", in order to explore the main factors affecting the quality uniformity of Danshen extract granules. Danshen standard decoction was prepared; then the fingerprint method was developed to determine the content of salvianolic acid B; and the main peaks in the fingerprint were identified with UPLC-QTOF/MS to clarify the chemical compositions of Danshen decoction. Three indexes were calculated to evaluate the stability of whole process, including the extraction ratio; transfer rate of index components and pH value. The results showed that the main components of Danshen decoction were phenolic acids, while the extraction rate, the transfer rate of salvianolic acid B and pH value were in a relatively stable level, and the similarity in the fingerprint of standard decoction was high, indicating that the preparation procedure was stable. The level of salvianolic acid B in the standard decoction was in a large range, which was mainly due to the difference in the quality of Salviae Miltiorrhizae Radix et Rhizoma.
