Simultaneous determination of eleven components in Ginkgo biloba leaves by high performance liquid chromatography method.
- Author:
Jin-Li LV
1
;
Biao YANG
1
;
Meng-Xuan LI
1
;
Zhao-Qing MENG
1
;
Shi-Ping MA
2
;
Zhen-Zhong WANG
1
;
Gang DING
1
;
Wen-Zhe HUANG
1
;
Wei XIAO
1
Author Information
- Publication Type:Journal Article
- Keywords: Ginkgo biloba leaves; bisflavonoids; flavonoid glycosides; high performance liquid chromatography; multi components determination
- From: China Journal of Chinese Materia Medica 2017;42(5):931-935
- CountryChina
- Language:Chinese
- Abstract: To study Ginkgo biloba leaves in different producing area, we establish an HPLC method for the simultaneously determination of seven flavonoids glycosides and four biflavonoids in G. biloba leaves. The analysis was performed on an Agilent ZORBAX SB-C₁₈ column(4.6 mm×250 mm, 5 μm) wich acetonitrile, and 0.4% phosphoric acid as mobile phase at flow rate of 1 mL•min⁻¹ in a gradient edution, and the detection was carried out at 254 nm.The calibration curves of the seven flavonoids glycosides and four biflavonoids had a good linearitiy with good recoveries. The established HPLC method is simple, rapid, accurate, reliable, and sensitive, and can be applied to the identification and quality control of G. biloba leaves.
