Evaluation of the hydroxynitrile lyase activity in cell cultures of capulin (Prunus serotina).
- Author:
Liliana HERNÁNDEZ
1
;
Héctor LUNA
;
Arturo NAVARRO-OCAÑA
;
Ma Teresa de Jesús OLIVERA-FLORES
;
Ivon AYALA
Author Information
1. Universidad Autónoma Metropolitana, Unidad Xochimilco, Depto. Sistemas Biológicos, A. P. 23/181, México, D. F., México. hevlilia@servidor.unam.mx
- Publication Type:Journal Article
- MeSH:
Acetonitriles;
metabolism;
Aldehyde-Lyases;
metabolism;
Benzaldehydes;
metabolism;
Biocatalysis;
Cells, Cultured;
Hydrogen Cyanide;
metabolism;
Nitriles;
metabolism;
Prunus;
cytology;
enzymology
- From:
Chinese Journal of Biotechnology
2008;24(7):1199-1201
- CountryChina
- Language:English
-
Abstract:
Enzymatic preparations obtained from young plants and cell cultures of capulin were screened for hydroxynitrile lyase activity. The three week old plants, grown under sterile conditions, were used to establish a solid cell culture. Crude preparations obtained from this plant material were evaluated for the transformation of benzaldehyde to the corresponding cyanohydrin (mandelonitrile). The results show that the crude material from roots, stalks, and leaves of young plants and calli of roots, stalks, internodes and petioles biocatalyzed the addition of hydrogen cyanide (HCN) to benzaldehyde with a modest to excellent enantioselectivity.
