Production and accumulation of xylooligosaccharides with long chains by growing culture and xylanase of a mutant strain of Bacillus pumilus X-6-19.
- Author:
Qingzhu YUAN
1
;
Tsuyoshi ADACHI
;
Shinji TAKENAKA
;
Shuichiro MURAKAMI
;
Machiko TANAKA
;
Kenji AOKI
Author Information
1. Division of Life Science, Graduate School of Science and Technology, Kobe University, Rokko, Kobe 657-8501, Japan.
- Publication Type:Journal Article
- MeSH:
Bacillus;
genetics;
metabolism;
Culture Techniques;
methods;
Endo-1,4-beta Xylanases;
biosynthesis;
genetics;
metabolism;
Glucose;
pharmacology;
Mutation;
Oligosaccharides;
biosynthesis;
chemistry;
genetics;
Recombinant Proteins;
biosynthesis;
genetics;
metabolism;
Soil Microbiology
- From:
Chinese Journal of Biotechnology
2008;24(7):1221-1227
- CountryChina
- Language:English
-
Abstract:
Bacillus pumilus X-6-9 isolated from soil and subsequently identified, produced xylooligosaccharides with long chains from xylan and accumulated them in the culture. By improving the culture conditions and mutating the bacterium, a 3.2-fold increase in the production of the xylooligosaccharides was established, when compared to the original culture conditions of B. pumilus X-6-19. The addition of D-glucose to the culture of the mutant strain U-3 of B. pumilus X-6-9 repressed the synthesis of beta-xylosidase, but not xylanase. Thus, it was revealed that strain U-3 was a good organism for the production and accumulation of xylooligosaccharides with long chains from xylan by a microbial culture. Xylanase produced by strain U-3 was purified to homogeneity and characterized. The hydrolyzates generated by the purified xylanase contained xylobiose, xylotriose, xylotetraose, and xylopentaose, but not xylose.
