Cloning and expression of the pig skeletal muscle musclin gene.
- Author:
Weijie WANG
1
;
Hongji LI
;
Liqiang HAN
;
Yueying WANG
;
Jing WANG
;
Weihua LI
;
Maowang LIN
;
Yulei TAI
;
Zhiqiang ZHANG
;
Meng ZANG
;
Yanling WANG
;
Guoyu YANG
Author Information
1. Laboratory ofAnimal Physiology & Biochemistry, He'nan Agricultural University, Zhengzhou 450002, China.
- Publication Type:Journal Article
- MeSH:
Amino Acid Sequence;
Animals;
Base Sequence;
Cloning, Molecular;
DNA, Complementary;
genetics;
Escherichia coli;
genetics;
metabolism;
Humans;
Mice;
Molecular Sequence Data;
Muscle Proteins;
biosynthesis;
genetics;
Muscle, Skeletal;
metabolism;
Open Reading Frames;
genetics;
Rats;
Recombinant Fusion Proteins;
biosynthesis;
genetics;
isolation & purification;
Swine;
genetics;
Transcription Factors;
genetics
- From:
Chinese Journal of Biotechnology
2008;24(7):1248-1252
- CountryChina
- Language:Chinese
-
Abstract:
We found seven tag sequence with high homology in dbEST by using human musclin gene, and got its cDNA sequence, which consists of 651bp and the open reading frame was 54-452 bp detected by RT-PCR, encoding 132 amino acid residue protein. The new gene has high homology with that of human, mouse and rat, the rate is 87.2%, 77.6% and 77.9%, respectively; the gene fragment was cloned into expression vector pGEX-4T-1, and the recombinant was transformed into E. coli BL21. Induced by IPTG, the fusion protein GST-musclin, a 38.59 kD protein was successfully expressed in E. coli BL21 and identified by Western blotting.
