Antiviral activity determination of recombinant equine interferon-gamma and identification inhibited antiviral activity of monoclonal antibodies.
- Author:
Yu BAI
1
;
Weiye CHEN
;
Tiegang TONG
;
Weijun ZHANG
;
Shulan XU
;
Qun WANG
;
Qingge SUN
;
Guangliang LIU
;
Zhigao BU
;
Donglai WU
Author Information
1. National Key Laboratory of Veterinary Biotechnology, Harbin Veterinary Research Institute, CAAS, Harbin 150001, China.
- Publication Type:Journal Article
- MeSH:
Animals;
Antibodies, Monoclonal;
immunology;
Antiviral Agents;
metabolism;
pharmacology;
Baculoviridae;
genetics;
metabolism;
Escherichia coli;
genetics;
metabolism;
Green Fluorescent Proteins;
metabolism;
Horses;
Interferon-gamma;
biosynthesis;
genetics;
pharmacology;
Recombinant Proteins;
Vesicular stomatitis Indiana virus;
drug effects;
metabolism
- From:
Chinese Journal of Biotechnology
2008;24(7):1258-1262
- CountryChina
- Language:Chinese
-
Abstract:
Equine interferon-gamma (eIFN-gamma) expressed both in E. coli and baculovirus were evaluated for antiviral activity against recombinant Vesicular Stomatits Virus expressing green fluorescence protein (rVSV-GFP) in EFK-78 cells. The assays were conducted in 96-well plate. Virus infectivity was measured by quantifying GFP-positive cells, instead of quantifying the CPE reduction. Prior to infection of EFK-78 cells with rVSV-GFP, the cells were incubated with eIFN-gamma. The GFP expression in the EFK-78 cells dramatically decreased in the cells treated with eIFN-gamma in a dose-dependent manner, comparing with the mock-treated cells. The titers of antiviral activity were 1 x 10(3) AU/mL and 1 x 10(5) AU/mL of eIFN-gamma expressed from E. coli and baculovirus, respectively. The antiviral activities of the recombinant eIFN-gamma were highly efficient and specific, as it was blocked by mAbs against eIFN-gamma.
