Transfecting keratinocyte and enhancing cell proliferation by integrated HPV11 DNA.
- Author:
Yi ZHANG
1
;
Fenglin LÜ
;
Caiyu CHEN
Author Information
1. Institute of Surgery and Daping Hospital, The Third Military Medical University, Chongqing 400042, China.
- Publication Type:Journal Article
- MeSH:
Cell Proliferation;
Cells, Cultured;
DNA, Viral;
genetics;
Genome, Viral;
genetics;
Human papillomavirus 11;
genetics;
Humans;
Keratinocytes;
cytology;
metabolism;
Open Reading Frames;
genetics;
Plasmids;
genetics;
Transfection
- From:
Chinese Journal of Biotechnology
2008;24(8):1367-1372
- CountryChina
- Language:Chinese
-
Abstract:
We constructed the plasmid containing the integrated open reading frame (ORF) HPV11 genome, and identified its function, to lay foundation for production of transgenic animal models of HPV11. Recombinant plasmid pQE-Trisystem- EGFP/HPV11 (pE/H) and pQE-Trisystem-EGFP/1.1 copy HPV11 (pE/1.1H) were constructed. Then, the human keratinocyte (KC) was transfected by pE/1.1H, pE/H and closed circular HPV11 and detected. The recombinant plasmid was successfully constructed. The expression of HPV11E6 gene was detected in the experimental group. Fluorescence was observed in the pE/1.1H and pE/H group. The HPV11, pE/H, and pE/1.1 enhanced the KC proliferation, with remarkable differences (P < 0.01) from the control group. Amongst the three experimental groups, pE/1.1H was found to be the weakest. The plasmid containing the integrated ORF of HPV11 (1.1 copy HPV11) genome was successfully constructed. The pE/1.1H had the same phenotype of wild-type HPV11 genome. It provided experimental material and methodological guidance for studying the low-risk HPV, as well as for the production of HPV11 transgenic mice.
