Generation and immunogenicity of a recombinant adenovirus expressing the E2 protein of classical swine fever virus in rabbits.
- Author:
Yuan SUN
1
;
Qiaofen QI
;
Bingbing LIANG
;
Dan CHENG
;
Na LI
;
Moyang YU
;
Yufei WANG
;
Nihong LIU
;
Qinghu ZHU
;
Huaji QIU
Author Information
1. Division of Swine Infectious Diseases, National Key Laboratory of Veterinary Biotechnology, Harbin Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Harbin 15000, China.
- Publication Type:Journal Article
- MeSH:
Adenoviridae;
genetics;
immunology;
metabolism;
Animals;
Antibodies, Viral;
blood;
Genetic Vectors;
genetics;
Immunization;
Rabbits;
Random Allocation;
Recombinant Proteins;
biosynthesis;
genetics;
immunology;
Transfection;
Viral Envelope Proteins;
biosynthesis;
genetics;
immunology;
Viral Vaccines;
immunology
- From:
Chinese Journal of Biotechnology
2008;24(10):1734-1739
- CountryChina
- Language:Chinese
-
Abstract:
Classical swine fever (CSF), which is caused by classical swine fever virus (CSFV), causes significant losses in pig industry in many countries in Asia and Europe. The E2 glycoprotein of CSFV is the main target for neutralizing antibodies. In this study, a recombinant replication-defective human adenovirus expressing the CSFV E2 gene (rAdV-E2) was generated and evaluated for the immunogenicity in rabbits. The results showed that the rabbits immunized with rAdV-E2 developed high-level CSFV-specific antibodies. The rAdV-E2-immunized rabbits were all free of the regular fever and the viral replication in the spleen upon challenge with C-strain, which were seen in the rabbits immunized with the parent adenovirus of rAdV-E2. This indicates that the recombinant adenovirus can be an attractive candidate vaccine against CSF.
