An enzyme electrode biosensor for inosine determination.
- Author:
Shiqing SUN
1
;
Jianguo SHI
;
Xuemei LI
;
Junhui YANG
;
Yaohong MA
;
Qingjun MENG
;
Dai SUN
Author Information
1. Adrrssed Institute of Biology, Shandong Academy of Sciences, Shandong Key Laboratory for Biosensors, Ji'nan 250014, China sundaiman77@yahoo.com.cn
- Publication Type:Journal Article
- MeSH:
Biosensing Techniques;
methods;
Inosine;
analysis;
Sensitivity and Specificity
- From:
Chinese Journal of Biotechnology
2008;24(10):1796-1800
- CountryChina
- Language:Chinese
-
Abstract:
An enzyme electrode biosensor was used for the amperometric determination of inosine in its tablets by co-immobilizing nucleoside phosporylase and xanthine oxidase on a hydrogen peroxide electrode. As a fundamental electrode the hydrogen peroxide electrode has an advantage of stability in analysis compared with the 02 electrode. The enzyme electrode showed a linear response to inosine in the range of 1-268 mg/L with a response of 60 seconds under a sample injection volume of 25 microL. Based on the enzyme electrode, inosine solutions were determined with an average recover rate of 100.8% and a relative standard deviation (RSD) of les than 0.14% in 20 assays. The lifetime of the enzyme electrode was relative long and could be used continuously at 25 degrees C for 25 days. These results demonstrated that the enzyme electrode biosensor could be used to determine inosine and its derivatives specifically, rapidly, conveniently and economically.
