DNA barcoding identification of endangered medicinal plants of Orchidaceae.
10.19540/j.cnki.cjcmm.2017.0090
- Author:
Huan TANG
1
;
Li XIANG
2
;
Xi-Wen LI
2
;
Wei SUN
2
;
Mei-Na WANG
3
;
Yun-Feng HUANG
4
;
Meng YE
1
Author Information
1. College of Forestry, Sichuan Agricultural University, Chengdu 611130, China.
2. Key Laboratory of Beijing for Identification and Safety Evaluation of Chinese Medicine, Institute of Chinese Materia Medica，China Academy of Chinese Medical Sciences, Beijing 100700, China.
3. The National Orchid Conservation Center of China and The Orchid Conservation & Research Center of Shenzhen, Shenzhen 518114, China.
4. Guangxi Institute of Chinese Medicine and Pharmaceutical Science, Nanning 530022, China.
- Publication Type:Journal Article
- Keywords:
DNA barcoding;
Orchidaceae;
medicinal plants;
molecular identification
- From:
China Journal of Chinese Materia Medica
2017;42(11):2058-2067
- CountryChina
- Language:Chinese
-
Abstract:
In this study, DNA barcoding was used to validate the traditional morphological classification of medicinal plants of Orchidaceae. The 163 samples of 135 species belong to 49 genera which have been confirmed by morphological identification were collected. Candidate sequences, including matK, psbA-trnH and ITS2 sequences, were amplified, bidirectionally sequenced, and assembled. All the sequences were blasted to GenBank database at NCBI, then analyzed using Neighbor-joining tree method by MEGA 7.0. The results showed that the DNAs of 163 samples were successfully extracted. The amplification efficiency of matK, psbA-trnH and ITS2 sequences were 100%, 100% and 98.77%, respectively. The 487 sequences were obtained, 345 sequences of which have matched corresponding sequences in the GenBank database and 142 sequences were new sequences. The topology of NJ tree which were constructed with the matK sequences was better than the trees of psbA-trnH and ITS2 sequences. In conclusion, the matK, psbA-trnH and ITS2 sequences were complementary and suitable for identification of medicinal plants of Orchidaceae. DNA barcoding can be used as an auxiliary means for identification of medicinal plants of Orchidaceae.
