Analyzing crude/processed root of Polygonum multiflorum from different habitats by UPLC fingerprint and mode identification methods.
10.19540/j.cnki.cjcmm.20170426.004
- Author:
Rong XIAO
1
;
Yan LIN
1
;
Si-Min LEI
1
;
Ying ZHANG
1
;
Jie HUANG
1
;
Bo-Hou XIA
1
;
Chun LI
2
;
Duan-Fang LIAO
1
;
Ping WU
1
;
Li-Mei LIN
1
Author Information
1. College of Pharmacy, Hunan University of Chinese Medicine, Changsha 410208, China.
2. Institute of Chinese Materia Medica, China Academy of Chinese Medical Sciences, Beijing 100700, China.
- Publication Type:Journal Article
- Keywords:
2，3，5，4′-tetrahydroxy stilbene-2-O-β-D-glucoside;
Polygonum multiflorum;
UPLC fingerprint;
cluster analysis;
principal components analysis
- From:
China Journal of Chinese Materia Medica
2017;42(12):2305-2310
- CountryChina
- Language:Chinese
-
Abstract:
To establish a content determination method for 2,3,5,4'-tetrahydroxy stilbene-2-O-β-D-glucoside (TSG) of the crude/processed root of Polygonum multiflorum from different habitats in China and set up the fingerprint by using UPLC. Various samples were pretreated by macro-porous resin. Then UPLC analysis was performed on Waters ACQUITY UPLC@BEH C18 chromatographic column (2.1 mm×50 mm, 1.7 μm) at (25±5) ℃. A binary gradient elution system was composed of acetonitrile (phase A) and 0.5% acetic acid solution (phase B). Detection was performed at the wavelength of 254 nm, and the mobile flow rate was set at 0.3 mL•min⁻¹. Results showed that the yield of extraction of the 2,3,5,4'-tetrahydroxy stilbene-2-O-β-D-glucoside from root of P. multiflorum was all over 25.0% after macro-porous resin separation; an exclusive UPLC fingerprint method of the crude/processed root of P. multiflorum from different habitats was successfully set up and 17 chromatographic peaks were calibrated. Cluster analysis can not entirely distinguish the crude one from the processed one, while principal component analysis absolutely can. 2,3,5,4'-tetrahydroxy stilbene-2-O-β-D-glucoside is the composition that has largest differences in variable importance in projection (VIP) between crude and processed root of P. multiflorum. The separating method can gain high-purity 2,3,5,4'-tetrahydroxy stilbene-2-O-β-D-glucoside, and the determination method is simple, sensitive, reliable and can be used in fast identifying the crude/processed root of P. multiflorum or as a method for overall quality control of root of P. multiflorum.
