Effect of oridonin on apoptosis and intracellular reactive oxygen species level in triple-negative breast cancer MDA-MB-231 cells.
10.19540/j.cnki.cjcmm.2017.0114
- Author:
Qi QI
1
;
Pei ZHANG
2
;
Qi-Xiang LI
2
;
Qiong PAN
2
;
Hai-Lun ZHENG
3
;
Su-Rong ZHAO
2
Author Information
1. Department of Basic Medical Sciences, Bengbu Medical College, Bengbu 233030, China.
2. School of Pharmacy, Bengbu Medical College, Bengbu 233030, China.
3. First Affiliated Hospital of Bengbu Medical College, Bengbu 233004, China.
- Publication Type:Journal Article
- Keywords:
apoptosis;
breast cancer;
oridonin;
reactive oxygen
- From:
China Journal of Chinese Materia Medica
2017;42(12):2361-2365
- CountryChina
- Language:Chinese
-
Abstract:
Oridonin, which is an ent-kaurene diterpenoid isolated from traditional Chinese medicine Rabdosia rubescens, displays various bioactivities, including anti-inflammation, anti-bacteria and anti-tumor. This study aimed to investigate the effect of oridonin on apoptosis of triple-negative breast cancer MDA-MB-231 cells and its underlying mechanisms. The inhibitory effect of oridonin on proliferation of MDA-MB-231 cells was measured by MTT assay; Apoptosis was analyzed by flow cytometry with PI staining and Annexin V-FITC/PI staining; Intracellular reactive oxygen species (ROS) level was determined by ROS detection kit, and expressions of PARP, Bcl-2, caspase-3 were analyzed by Western blot. The results showed that oridonin exhibited a significant effect in inducing apoptosis of MDA-MB-231 cells, enhancing intracellular ROS level, down-regulating expression of Bcl-2 protein, and promoting cleavage of caspase-3 and its substrate PARP. These results indicated that the apoptosis-inducing effect of oridonin on MDA-MB-231 cells might be correlated with increase of intracellular ROS level, down-regulation of Bcl-2 protein and activation of caspase-3.
