Ghrelin stimulates in vitro angiogenic capacity of rat cardiac microvascular endothelial cells
10.3760/cma.j.issn.0253-3758.2012.01.011
- VernacularTitle:生长激素促分泌物受体的内源性配体ghrelin促进大鼠心肌微血管内皮细胞体外血管生成
- Author:
Li WANG
1
;
Qing-Wei CHEN
;
Gui-Qiong LI
;
Da-Zhi KE
Author Information
1. 重庆医科大学附属第二医院
- Keywords:
Neovascularization,physiologic;
Ghrelin
- From:
Chinese Journal of Cardiology
2012;40(1):50-56
- CountryChina
- Language:Chinese
-
Abstract:
Objective To clarify whether ghrelin could promote in vitro rat cardiac microvascular endothelial cells(CMECs)angiogenesis and related mechanisms.Methods CMECs were isolated from myocardial tissue of adult male SD rats and characterized by the immunocytochemistry staining with Factor Ⅷ and the capacity of in vitro capillary tube-like formation.The mRNAs and protein expressions of ghrelin and its receptor(growth hormone secretagogue receptor,GHS-R)of CMECs were determined by RT-PCR,Immunofluorescence,ELISA and Western blot.Proliferation,migration and in vitro angiogenesis as well as ERK2 phosphorylation of CMECs were tested in the presence of ghrelin(10-9-10-7 mol/L)with or without pretreatment with specific MAPK/ERK2 inhibitor PD98059.Results Purity of CMECs characterized by immunocytochemistry staining with Factor Ⅷ was about 95％,and the cells showed a high ability to form the capillary tube-like structures on Matrigel.Ghrelin and GHS-R were constitutively expressed in CMECs.Proliferation,migration and in vitro angiogenesis capacities of CMECs(72.20 ±5.72 vs.28.60 ±5.13,P ＜0.001;71.00±7.78 vs.28.60 ±5.13,P ＜0.001)as well as ERK2 phosphorylation(0.92 ±0.13 vs.0.29 ± 0.04,P ＜ 0.001 ; 1.15 ± 0.16 vs.0.29 ± 0.04,P ＜ 0.001)were significantly enhanced by exogenous ghrelin(10-8-10-7 mol/L).PD98059 abolished ghrelin-induced ERK2 phosphorylation and in vitro angiogenesis.Conclusions Ghrelin and its receptor are expressed in CMECs and ghrelin could stimulate CMECs in vitro angiogenesis through activation of MAPK/ERK2 signaling pathway.
