Role of C-type natriuretic peptide receptor and large-conductance calcium-activated potassium channels in brain natriuretic peptide-induced porcine coronary artery dilation
10.3760/cma.j.issn.0253-3758.2012.02.016
- VernacularTitle:利钠肽C受体和大电导钙激活钾通道在B型利钠肽舒张猪冠状动脉中的作用
- Author:
Qi-Yong LI
1
;
Rong-Jian JIANG
;
Yan SHU
;
Hong KONG
;
Jin-Chuan LAI
;
Biao CHENG
Author Information
1. 四川省医学科学院四川省人民医院
- Keywords:
Coronary vessels;
Large-conductance calcium-activated potassium channels;
Natriuretic peptide,brain;
Patch-clamp techniques
- From:
Chinese Journal of Cardiology
2012;40(2):157-160
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the role of C-type natriuretic peptide receptor (NPR-C) and large-conductance calcium-activated potassium channels ( BKCa ) in brain natriuretic peptide (BNP) induced porcine coronary artery dilation.Methods Porcine coronary artery rings were obtained and treated with BNP (10-6 mol/L), BNP + NPR-C antagonist cANF4-28 (10-6 mol/L) and BNP + BKCa blocker tetraethylammonium (TEA, 1 mmol/L). The vascular tone experiments were observed on 10 vessel segments. BKCa current density was measured by the whole-cell patch clamp technique.Results The maximum diastolic rate was similar between BNP group ( 68.51％ ± 11.50％ ) and cANF4-28 + BNP group (65.67％ ± 11.90％,P ＞ 0.05) while significantly reduced in TEA + BNP group (28.87％ ± 4.55％,all P ＜ 0.05 ).When the holding potential was set at + 60 mY,the BKCa current density of BNP group was (78.48 ±5.86) pA/pF,which was significantly higher than control group [ (53.84 ±4.55) pA/pF,P ＜0.05],which was equally reduced in the TEA group and TEA + BNP group [ (28.80 ± 2.76) pA / pF and (30.60 ± 3.88) pA/pF respectively,all P ＜ 0.05 vs.control group ].Conclusion BNP could relax the porcine coronary arterial smooth muscles by increasing BKca current,and this effect is not mediated by NPR-C.
