MicroRNAs expression in normal and dissected aortic tissue.
- Author:
Zi-yang HU
1
;
Jian-fang LUO
;
Shi-long ZHONG
;
Ling XUE
;
Yi-fei CHEN
;
Rui-xin FAN
Author Information
- Publication Type:Journal Article
- MeSH: Aneurysm, Dissecting; genetics; Gene Expression Profiling; Humans; MicroRNAs; genetics; metabolism; Oligonucleotide Array Sequence Analysis; Reverse Transcriptase Polymerase Chain Reaction
- From: Chinese Journal of Cardiology 2012;40(5):406-410
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVESTo investigate if there is altered microRNAs (miRNAs) expression in aortic dissection (Debakey Type A) and normal aorta tissue.
METHODSTotal RNA was exacted from aorta of 5 patients with aortic dissection (AD) and four patients without aortic diseases (NA). miRNAs of the aortic tissues were analyzed by miRNA microarray. Reverse transcription polymerase chain reaction (RT-PCR) was performed to verify the expression of miRNAs in larger sample size (AD = 11 and NA = 9).
RESULTShsa-miR-146b-5p_st, hsa-miR-19a_st and hsa-miR-505_st were significantly upregulated while hsa-miR-1268_st and hsa-miR-939_st were significantly downregulated [fold change > 2, q-value (%) ≤ 5] in AD group compared with NA group. RT-PCR verified hsa-miR-146b-5p_st miRNAs change in AD group.
CONCLUSIONSAltered miRNAs expression might play an essential role in the pathogenesis of aortic dissection formation and hsa-miR-146b-5p_st might serve as a new diagnosis biomarker of aortic dissection.
