MicroRNAs expression in normal and dissected aortic tissue

VernacularTitle:应用基因芯片初步分析主动脉夹层与正常主动脉微小RNA的差异表达
Author: Zi-Yang HU ¹ ; Jian-Fang LUO ; Shi-Long ZHONG ; Ling XUE ; Yi-Fei CHEN ; Rui-Xin FAN
Author Information

1. 广东省心血管病研究所广东省医学科学院广东省人民医院
Keywords: Aneurysm,dissecting; Microchip anglytical procedures; Reverse transcriptase polymerase chain reaction
From: Chinese Journal of Cardiology 2012;40(5):406-410
CountryChina
Language:Chinese
Abstract: Objectives To investigate if there is altered microRNAs (miRNAs) expression in aortic dissection ( Debakey Type A) and normal aorta tissue.Methods Total RNA was exacted from aorta of 5 patients with aortic dissection(AD) and four patients without aortic diseases (NA).miRNAs of the aortic tissues were analyzed by miRNA microarray.Reverse transcription polymerase chain reaction (RT-PCR) was performed to verify the expression of miRNAs in larger sample size ( AD =11 and NA =9 ).Results hsa-miR-146b-5p_st,hsa-miR-19a_st and hsa-miR-505_st were significantly upregulated while hsa-miR-1268_st and hsa-miR-939_st were significantly downregulated [fold change ＞ 2,q-value (％) ≤5 ] in AD group compared with NA group.RT-PCR verified hsa-miR-146b-5p_st miRNAs change in AD group.Conclusions Altered miRNAs expression might play an essential role in the pathogenesis of aortic dissection formation and hsa-miR-146b-5p_st might serve as a new diagnosis biomarker of aortic dissection.