Expression of extracellular matrix metalloproteinase inducer in the unstable plaque of patients with acute coronary syndrome
10.3760/cma.j.issn.0253-3758.2012.05.013
- VernacularTitle:白三烯B4和细胞外基质金属蛋白酶诱导因子与急性冠状动脉不稳定斑块关系的实验研究
- Author:
Bin WANG
1
;
Sha-Sha XU
;
Jian-Jun JIANG
;
Xian-Ben LU
;
Ying-Sheng XUE
;
Jiao-Chen WANG
;
Ya-Fei MI
;
Min ZHU
;
Wei-Li GE
;
Li-Jiang TANG
Author Information
1. 温州医学院附属医院浙江省台州医院
- Keywords:
Coronary disease;
Leukotriene B4;
Macrophages;
Extracellular matrix metalloproteinase inducer
- From:
Chinese Journal of Cardiology
2012;40(5):416-420
- CountryChina
- Language:Chinese
-
Abstract:
Objective To observe the expression of extracellular matrix metalloproteinase inducer (EMMPRIN) in the unstable plaque of patients with acute coronary syndrome (ACS),and the impact of leukotriene B4 (LTB4) on the EMMPRIN expression in macrophages.Methods The EMMPRIN expression was detected by immunohistochemistry in 11 unstable plaques from patients with ACS.Protein expression of EMMPRIN was evaluated by Western blot on macrophages differentiated from THP-1 which were stimulated with LTB4 in the absence or presence of LTB4 antagonist U75302.There are 8 study groups:1-THP-1,2 -8-the macrophages derived from THP-1,2 - 6- macrophages were stimulated by LTB4 (0,10-10,10-9,10-8 and 10-7mol/L) for 24 h,7 -8-the macrophages were pretreated by 10-6 mol/L or 10-7 mol/L U75302 2 h before the LTB4 ( 10-7 mol/L) stimulation.Results Abundant EMMPRIN expression was detected in macrophages and smooth muscle cells of unstable plaques from ACS patients.As to the THP-1 derived macrophages,EMMPRIN expression was significantly upregulated in a concentration-dependent manner in LTB4 stimulated groups,which was significantly higher in group 3 -6 than in the THP-1 group ( group 1 ) and macrophages group ( group 2 ) ( all P ＜ 0.05 ) and pretreatment with U75302 significantly reduced the LTB4 induced upregulation of EMMPRIN in a dose-dependent manner (P ＜ 0.05).Conclusion EMMPRIN expression is enhanced in macrophages and smooth muscle cells on unstable coronary artery plaques from ACS patients.LTB4 could stimulate EMMPRIN expression on THP-1 derived macrophages suggesting that LTB4 and EMMPRIN might be both involved in the formation and progression of unstable plaques,future studies are warranted to explore if LTB4 and EMMPRIN antagonists are effective or not for treating patients with ACS.
