Determination of verbascoside in herba of Galeobdolon Chinense by RP-HPLC.
- Author:
Shou-jun JIANG
1
;
Bin ZHU
;
Rui-chao LIN
;
Zun-jian ZHANG
Author Information
- Publication Type:Journal Article
- MeSH: Chromatography, High Pressure Liquid; methods; Glucosides; analysis; Lamiaceae; chemistry; Phenols; analysis; Plants, Medicinal; chemistry
- From: China Journal of Chinese Materia Medica 2002;27(4):263-265
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo determine Verbascoside in Galeobdolon chinense.
METHODHPLC method was used, with the column packed with Kromasil C18(4.6 mm x 150 mm, 5 microns). The mobile phase was a mixture of acetonitrile and 1% acetic acid in a ratio of 13:87, and the ultraviolet wavelength was set at 350 nm.
RESULTThe average recovery was 97.0%, the RSD being 1.69%.
CONCLUSIONThis method is sensitive and reliable. It can be used to determine being Verbascoside in Galeobdolon chinense.
