Qualitative and quantitative methods of chlorogenic acid in commercial Herba Artemisiae Scopariae.
- Author:
Xiao-jun SONG
1
;
Yong-xin ZHANG
;
Ying ZHANG
;
Qi-wei ZHANG
Author Information
- Publication Type:Journal Article
- MeSH: Artemisia; chemistry; Chlorogenic Acid; analysis; Plants, Medicinal; chemistry; Quality Control
- From: China Journal of Chinese Materia Medica 2002;27(4):267-269
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo develop the identification and assay for chlorogenic acid in commercial Herba Artemisiae Scopariae.
METHODTLC method was used for identification with silica gel G plate and butyl acetate-formic acid-water (7:2.5:2.5) upper layer as a developing solvent. Chlorogenic acid in 85% methanolic extract was separated on the ODS column with methonal-3% acetic acid solution (15:85) as mobile phase. The detection wavelength was 327 nm.
RESULTThe qualitative method is repeatable. Chlorogenic acid in extracts is well separated, relationship of injection amount and peak area is linear (r = 0.9998) within the range of 0.075-0.6 microgram. The average recovery is 100.9% and repeatability is 1.4%. Ten samples purchased from different areas in the country were identified and quantified with the methods.
CONCLUSIONThe methods and data could be used for quality control.
