Influence of the watery extract of jiangzhining decoction on the genetic expression of hepatocyte LDLR of hyperlipidemic rats.
- Author:
Jian-hong GUAN
1
;
Ai-hua LIANG
;
Qian-jin FENG
;
Shan-shan HU
Author Information
- Publication Type:Journal Article
- MeSH: Actins; biosynthesis; genetics; Animals; Drug Combinations; Drugs, Chinese Herbal; administration & dosage; pharmacology; Hyperglycemia; metabolism; Hypoglycemic Agents; administration & dosage; pharmacology; Liver; metabolism; Male; RNA, Messenger; genetics; Rats; Rats, Wistar; Receptors, LDL; biosynthesis; genetics
- From: China Journal of Chinese Materia Medica 2002;27(4):289-301
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo study the influence of Jiangzhining decoction on the genetic expression of Liver LDLR of the rats suffered from hyperlipemia.
METHODLaboratory animals were male wister rats with hyperlipemia resulting from high fat feeding. Prescription was the douche of stomach with Jiangzhining decoction (200%) with a dosage of 1.4 g.kg-1, for 15 successive days. Total RNA was extracted from the liver tissue of treated rats and LDLRmRNA was detected by Dot blot hybridization. Expression levels of LDLRmRNA was estimated by a ratio of LDLRmRNA and beta-actin mRNA.
RESULTThe difference between expression levels of LDLRmRNA for normal group and those for hyperlipemia group (100% +/- 19% vs 39% +/- 14%) was significant (P < 0.05); and the difference between decoction group (108 +/- 8%) and hyperlipimia group was also highly significant (P < 0.01).
CONCLUSIONHigh fat feeding reduces the expression of liver LDLRmRNA while the decoction can greatly increase it. The study and development of Jiangzhining are significant in preventing and curing cadiocerebral diseases.
