Determination of gossypol in cotton root bark by HPLC.
- Author:
Guang-hong CUI
1
;
Jia-chun CHUN
;
Da-yong CAI
Author Information
- Publication Type:Journal Article
- MeSH: Chromatography, High Pressure Liquid; Gossypium; chemistry; Gossypol; analysis; Plant Roots; chemistry; Plants, Medicinal; chemistry
- From: China Journal of Chinese Materia Medica 2002;27(3):173-175
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo establish a method for the determination of gossypol in cotton root bark.
METHODSamples were extracted with acetone, and determined on a C18 column with the mobile phase (Acetonitrile-0.2% phosphoric acid, 85:15) and UV-235 nm detector.
RESULTThe recovery rate was 97.6%, RSD 1.59% (n = 5).
CONCLUSIONThis method can be used for the determination of gossypol in cotton root bark and the content of gossypol in three different species has been determined.
