Determination of curculigoside in crude medicine Curculigo orchioides by HPLC.
- Author:
Hui-wen LU
1
;
Bing-hui ZHU
;
Ying-kang LIANG
Author Information
- Publication Type:Journal Article
- MeSH: Benzoates; analysis; Chromatography, High Pressure Liquid; Curculigo; chemistry; Glucosides; analysis; Plants, Medicinal; chemistry; Quality Control; Rhizome; chemistry
- From: China Journal of Chinese Materia Medica 2002;27(3):192-194
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo establish a method for the determination of curculigoside in Curculigo orchioides, a species of crude medicine.
METHODThe methanol was used as the solvent of exatraction and the curculigoside was extracted from the crude medicine with the method of the ultrasonic vibration. Using Sep-Pak C18 cartridges to purify the solution, the curculigoside was detected by HPLC. Intersil ODS-3(150 mm x 4.6 mm, 5 microns) chromatographic column was used, mobile phase of methanol-water-ice acetic acid (45:80:1) and detect wavelength was set at UV 283 nm.
RESULTThe average recovery was 99.2% for the determination of curculigoside, RSD = 1.7% (n = 5). The content range of the curculigoside for 6 kinds of different samples was from 0.11% to 0.35%.
CONCLUSIONThis method could be used to control the quality of crude medicine C. orchioides.
