Determination of trigonelline in Trigonella foenum-graecum by HPLC.
- Author:
Huai-qing ZHAO
1
;
Yan QU
;
Xue-ya WANG
;
Hong-jian ZHANG
;
Fa-mei LI
;
Hattori MASAO
Author Information
- Publication Type:Journal Article
- MeSH: Alkaloids; analysis; Chromatography, High Pressure Liquid; Plants, Medicinal; chemistry; Quality Control; Seeds; chemistry; Trigonella; chemistry
- From: China Journal of Chinese Materia Medica 2002;27(3):194-196
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVEA HPLC method is established to determine the content of trigonelline in Trigonella foenum-graecum.
METHODThe medicinal material was extracted by petholeum ether-ethanol. Asahipak NH2P-50 column was used, mobilephase consisted of acetonitrile-water(75:25) and detection wavelength was set at UV 265 nm.
RESULTThe standard curve was linear in the range of 3.68-73.60 micrograms.mL-1 with the correlation coefficient of 0.9999. The average recovery rate and RSD were 97.4% and 1.83% (n = 6) respectively.
CONCLUSIONIt provides scientific indexes for quality control of T. foenum-graecum.
