Molecular authentication of Dendrobium chrysanthum from its allied species of Dendrobium.
- Author:
Xiao-yu DING
1
;
Luo-san XU
;
Zheng-tao WANG
;
Hong XU
;
Kai-ya ZHOU
Author Information
- Publication Type:Journal Article
- MeSH: Base Sequence; DNA, Plant; genetics; Dendrobium; classification; genetics; Drug Contamination; Molecular Sequence Data; Plants, Medicinal; genetics; Sequence Analysis, DNA; Species Specificity
- From: China Journal of Chinese Materia Medica 2002;27(6):407-411
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo define molecular characters to distinguish D. chrysanthum from its allied species D. primulinum, D. lituiflorum, D. aphyllum, D. crepidatum.
METHODThe molecular characteristics of D. chrysanthum and its allied species were compared. The sequences of rDNA ITS regions were exploited to explore the evidence for authentication D. chrysanthum and its allied species.
RESULTAlthough the morphological difference was slight, the sequence difference of ITS regions among five rDNAs was obvious and stable. Fifteen sites of ITS region were defined as DNA character to identify D. chrysanthum from the other four allied species.
CONCLUSIONThe difference of rDNA ITS sequences can be used to authenticate accurately D. chrysanthum from three allied species of Dendrobium.
