Studies on anti-endotoxin activity of F022 from Radix Isatidis.
- Author:
Ai-hua LIN
1
;
Shu-xian FANG
;
Jian-guo FANG
;
Guang DU
;
Yun-hai LIU
Author Information
- Publication Type:Journal Article
- MeSH: Animals; Drugs, Chinese Herbal; isolation & purification; pharmacology; Female; Fever; chemically induced; drug therapy; Interleukin-6; secretion; Isatis; chemistry; Lipopolysaccharides; antagonists & inhibitors; Macrophages, Peritoneal; secretion; Male; Mice; Mice, Inbred C57BL; Plant Roots; chemistry; Plants, Medicinal; chemistry; Rabbits; Tumor Necrosis Factor-alpha; secretion
- From: China Journal of Chinese Materia Medica 2002;27(6):439-442
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo study the anti-endotoxin activity and mechanism of F022 from Radix Isatidis.
METHODThe production of TNF-alpha and IL-6 of murine peritoneal macrophages stimulated by LPS was measured by ELISA. The temperature in rabbits was tested after i.v. administration of LPS. The lethality of BCG-primed mice was induced by LPS.
RESULTIf F022 was added to macrophages culture simultaneously with LPS or 1 h before addition of LPS, production of TNF-alpha and IL-6 by macrophages was remarkably inhibited in vitro. F022 inhibited the fever induced by LPS in rabbits and protected BCG-primed mice from LPS induced lethality if given before administration of LPS.
CONCLUSIONThe anti-endotoxin effect of F022 may inhibit LPS binding to its receptor, and it may be a LPS receptor antagonist.
