Antagonistic effect of aqueous extract of detoxified cottonseeds on corticosterone-induced lesion in cultured PC12 cells.
- Author:
Yun-feng LI
1
;
Ming YANG
;
Yi-min ZHAO
;
Xin-hui LUAN
;
Zhi-pu LUO
Author Information
- Publication Type:Journal Article
- MeSH: Adenylyl Cyclases; metabolism; Animals; Anti-Anxiety Agents; pharmacology; Antidepressive Agents; pharmacology; Corticosterone; antagonists & inhibitors; Drugs, Chinese Herbal; isolation & purification; pharmacology; Gossypium; chemistry; Neuroprotective Agents; pharmacology; PC12 Cells; drug effects; Plants, Medicinal; chemistry; Rats; Rats, Wistar; Seeds; chemistry; Synaptic Membranes; enzymology
- From: China Journal of Chinese Materia Medica 2002;27(6):442-446
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo study the possible mechanism of aqueous extract of detoxified cottonseeds (CTN-W).
METHOD AND RESULTCTN-W 0.01, 0.03, 0.10, 0.30 mg.mL-1 was incubated directly with the synaptic membrane extracted from the cerebral cortex in rats, and adenylyl cyclase (AC) activity was detected by using radio-immunoassay.
RESULTShowed that CTN-W could activate AC in a dose-dependend manner. After incubation with PC12 cells in the presence of corticosterone 2 x 10(-4)mol.L-1 for 48 h, CTN-W 0.08, 0.4, 2 mg.mL-1 protected PC12 cells from the lesion induced by corticosterone.
CONCLUSIONAntidepressant and anxiolytic effects of CTN-W are related with the activation of AC-cAMP pathway in signal transduction system, thus protecting neurons from the lesion. These two aspects maybe partly form the mechanism of CTN-W's action.
