Research on the extracorporeal cytocompatibility of a composite of HA, carbon fiber and polyetheretherket-one.
- Author:
Xueyong LIU
1
;
Chunbo DENG
;
Jiquan LIU
;
Jianjun LI
;
Guoxin SUI
Author Information
1. Department of Orthopedic Surgery, Shengjing Hospital Affiliated to China Medical University, Shenyang 110004, China. liuxy@sj-hospital.org
- Publication Type:Journal Article
- MeSH:
Alkaline Phosphatase;
metabolism;
Animals;
Biocompatible Materials;
Carbon;
chemistry;
Cell Proliferation;
drug effects;
Cells, Cultured;
Durapatite;
chemistry;
Ketones;
chemistry;
Orthopedics;
Osteoblasts;
chemistry;
Polyethylene Glycols;
chemistry;
Prostheses and Implants;
Rats
- From:
Journal of Biomedical Engineering
2011;28(6):1159-1164
- CountryChina
- Language:Chinese
-
Abstract:
The present research was to study the biocompatibility of a composite of hydroxyapatite (HA), carbon fiber (CF) and polyetheretherket-one (PEEK) by co-culturing with the osteoblasts in vitro. Cell relative growth (RGR) was used as a quantitative assessment for cytotoxicity of the biomaterials by CCK-8. The proliferation index of the co-cultured cells and ALP activity was measured to study the effect of PEEK-HA-CF composites. Morphological properties of the osteoblast cells in vitro were observed by scanning electro-microscopy (SEM). The PEEK-HA-CF materials have no cytotoxicity to osteoblasts. The proliferation index of PEEK-HA-CF was higher than that of Ti alloy group, but these was no significant difference compared to that of control group. The ALP activity was the highest on PEEK-HA-CF composites surface after 7 days. The osteoblast cells co-cultured with the PEEK-HA-CF composite were adhered well to the biomaterial as observed under the SEM. The results suggested that the PEEK-HA-CF composites had good biocompatibility in vitro and might be a novel orthopedic implanted material.
