In vitro experimental study of rat cardiomyocyte injury with targeting of perfluorocarbon lipid particles.
- Author:
Baiyong HE
1
;
Zhaohuan LI
;
Hong TANG
;
Guohua LI
;
Song CHEN
;
Lian WANG
;
Haibo SONG
;
Hua FANG
;
Jun ZENG
Author Information
1. Department of Cardiology, West China Hospital, Sichuan University, Chengdu 610041, China.
- Publication Type:Journal Article
- MeSH:
Animals;
Animals, Newborn;
Antibodies, Monoclonal;
metabolism;
Cells, Cultured;
Contrast Media;
Female;
Fluorocarbons;
immunology;
metabolism;
Intercellular Adhesion Molecule-1;
metabolism;
Lipids;
chemistry;
Male;
Microspheres;
Myocytes, Cardiac;
metabolism;
pathology;
Rats;
Rats, Sprague-Dawley;
Tumor Necrosis Factor-alpha;
pharmacology;
Ultrasonography
- From:
Journal of Biomedical Engineering
2011;28(6):1170-1174
- CountryChina
- Language:Chinese
-
Abstract:
The present study was to investigate in vitro the rat cardiomyocyte injury with targeting of home-made perfluorocarbon lipid particles with avidin-biotin interaction. Neonatal rat cardiomyocytes were cultured in vitro and divided into two groups: TNF-alpha activated group and non-activated group. Those in the TNF-alpha activated group were exposed to 200 ng/ml TNF-alpha solution for 6 hours and then cardiomyocytes in both groups were pretargeted with biotinylated ICAM-1 monoclonal antibodies, and were exposed to streptavidin, and then to homemade green fluorescently-labeled biotinylated perfluorocarbon lipid particles. Cardiomyocytes nucleus stained with Hoechst. The results were detected with fluorescence microscope. As a result, in TNF-alpha activated group, around blue fluorescent cardiomyocytes nucleus, a great amount of green fluorescent particles were found, while there were few green fluorescent particles in non-TNF activated group. It has been shown that ICAM-1 is expressed in the surface of cardiomyocytes when they are stimulated by TNF-alpha. Perfluorocarbon lipid particles associated with ICAM-1 monoclonal antibodies can be targeted to injured cardiomyocytes by avidin-biotin interaction.
