Acqusition of full-length gene for rabbit osteoprotegerin.
- Author:
Chuanxiu SUN
1
;
Wenzhi ZHAO
;
Shengwei HE
;
Xu FANG
Author Information
1. The North Hospital, No. 2 Affiliated Hospital, Dalian Medical University, Dalian 116031, China.
- Publication Type:Journal Article
- MeSH:
Animals;
Base Sequence;
Molecular Sequence Data;
Osteoprotegerin;
genetics;
Rabbits;
Sequence Homology, Amino Acid
- From:
Journal of Biomedical Engineering
2012;29(1):116-120
- CountryChina
- Language:Chinese
-
Abstract:
This paper is to show a way of acqusition of the variable region gene of rabbit osteoprotegerin (OPG) and to analyse series. Total RNA was extracted from rabbit tibia, transcripted reversely into cDNA with random primers. The variable region of the OPG gene ampliflied using 5'RACE. Sequencing was confirmed by agarose gel electrophoresis and sequencing analysis. Full length of OPG gene was 1540bp that encoding 400 amino acids. It shared 89% identity with human OPG in whole amino acid sequence and about 85% with rattus norvegicus and other mammal. The OPG sequence of rabbit was obtained by 5'RACE, which could provide a good basis for OPG functional study.
