Effects of benazepril on apoptosis in the kidney of diabetic rats.
- Author:
Hui-jun DUAN
1
;
Yan-ling ZHANG
;
Yong-hong SHI
;
Fang LIU
;
Ying-min LI
Author Information
- Publication Type:Journal Article
- MeSH: Angiotensin-Converting Enzyme Inhibitors; pharmacology; Animals; Apoptosis; Benzazepines; pharmacology; Diabetes Mellitus, Experimental; metabolism; pathology; Diabetic Nephropathies; metabolism; pathology; Fas Ligand Protein; Kidney; metabolism; pathology; Male; Membrane Glycoproteins; metabolism; Random Allocation; Rats; Rats, Sprague-Dawley; fas Receptor; metabolism
- From: Acta Pharmaceutica Sinica 2002;37(6):409-411
- CountryChina
- Language:Chinese
-
Abstract:
AIMTo investigate the effects of angiotensin converting enzyme inhibitor (ACEI) on apoptosis and the expression of Fas and Fas-L in the kidney of diabetic rats.
METHODSUninephrectomized Spraque-Dawley rats were used to induce diabetes by intraperitoneal injection of streptozotocin (65 mg.kg-1). Benazepril (10 mg.kg-1) was given daily by gavage from the next day of the induction to diabetes for 12 weeks. Apoptosis was evaluated by means of terminal-deoxynucleotidyl transferase mediated d-UTP nick end labeling (TUNEL). Flow cytometry and immunohistochemistry were used to detect the expression of Fas and Fas-L.
RESULTSCompared with those in the kidneys of control group, apoptotic cells were more in number and the expression of Fas and Fas-L was higher in the diabetic kidneys (P < 0.05). The number of apoptotic cells and the level of expression of Fas and Fas-L were reduced by benazepril treatment (P < 0.05).
CONCLUSIONAngiotensin converting enzyme inhibitor benazepril showed some renal protective effect on diabetic nephropathy, partly through inhibiting apoptosis by down-regulating Fas and Fas-L expression.
