Study on pharmaceutical characterization and pharmacokinetics of daunorubicin long-circulating liposomes in rat.
- Author:
Hua ZHANG
1
;
Xian-rong QI
;
Qiang ZHANG
Author Information
- Publication Type:Journal Article
- MeSH: Animals; Antibiotics, Antineoplastic; administration & dosage; pharmacokinetics; Daunorubicin; administration & dosage; pharmacokinetics; Delayed-Action Preparations; Drug Carriers; Liposomes; Male; Phosphatidylethanolamines; Polyethylene Glycols; chemistry; Random Allocation; Rats; Rats, Wistar
- From: Acta Pharmaceutica Sinica 2002;37(4):299-303
- CountryChina
- Language:Chinese
-
Abstract:
AIMTo study the pharmaceutical characterization and pharmacokinetics of long-circulating liposomes containing daunorubicin.
METHODSThe morphology of daunorubicin long-circulating liposome was surveyed under the transmission electron microscope. The size of daunorubicin long-circulating liposomes was determined by laser scatter method. The entrapment efficiency and accelerative experiment stability of the daunorubicin long-circulating liposomes were examined. Visible spectrophotometry and the HPLC method were established for determination of the daunorubicin in the long-circulating liposomes. The percent release of daunorubicin from long-circulating liposomes in HBS (pH 7.5) and rat serum at 37 degrees C were examined. The pharmacokinetics in rats were studied.
RESULTSThe high entrapment efficiency (> 85%) and stabilized long-circulating liposomes could be achieved. The drug was slowly released from the daunorubicin long-circulating liposomes. The drug released from liposomes was less than 10% in 24 h. The T1/2 alpha and AUC of long-circulating liposome were higher than those in injections.
CONCLUSIONThe long-circulating liposomes prepared by us have high encapsulation efficiency and the pharmaceutical characterization showed good stability, they can be used for clinical purpose.
