Establishment of immune insulin resistance model in the rats by i.v. injection of BCG.
- Author:
Yu-xiao GUO
1
;
Mou-lun LUO
;
Zhi-bin LIN
Author Information
- Publication Type:Journal Article
- MeSH: Animals; Blood Glucose; metabolism; Diabetes Mellitus; metabolism; Glucose Clamp Technique; Glucose Tolerance Test; Injections, Intravenous; Insulin; blood; Insulin Resistance; immunology; Male; Mycobacterium bovis; Random Allocation; Rats; Rats, Wistar; Spleen; pathology; Tumor Necrosis Factor-alpha; metabolism
- From: Acta Pharmaceutica Sinica 2002;37(5):321-325
- CountryChina
- Language:Chinese
-
Abstract:
AIMTo observe the stability of BCG-induced insulin resistance model.
METHODSThe glucose tolerance, serum glucose, FFA, insulin, triglycerides, cholesterol, TNF-alpha and ALT level were measured. The change of GDR was measured by euglycemic clamp in model rats after given i.v. BCG 2, 4 and 8 weeks.
RESULTSAfter 2, 4 and 8 weeks, the GIR and glucose tolerance of the animals deceased significantly. After 2, 4 and 8 weeks, BCG infusion resulted in a pronounced reduction in glucose tolerance and insulin-stimulated glucose disposal rate [GDR = GDR: (29 +/- 6) vs (13 +/- 7) mg.kg-1.min-1 2 weeks; (29 +/- 6) vs (11 +/- 7) mg.kg-1.min-1 4 weeks and (23 +/- 3) vs (16 +/- 3) mg.kg-1.min-1 8 weeks, respectively, P < 0.01]. BCG infusion resulted in a pronounced increase in the weights of the liver [(6.2 +/- 0.9) vs (8.2 +/- 1.3) g, P < 0.05] and spleens [(0.51 +/- 0.11) vs (1.4 +/- 0.4) g, P < 0.01]. The histo-pathological results showed that BCG infusion resulted severe inflammation in the livers and spleens and the ratio of beta/alpha in pancreas increased. The serum levels of triglyceride, FFA and glucose were unchanged, but the level of serum TNF-alpha [543 +/- 60) vs (759 +/- 137) pg.mL-1, P < 0.05] and insulin [(31 +/- 5) vs (36 +/- 5) mu.L-1, P > 0.05] increased.
CONCLUSIONThis novel model of immune insulin resistance is completely and constantly established.
