Effect of recombinant prothymosin alpha on secretion of IFN-gamma, IFN-alpha and TNF-alpha in vitro.
- Author:
Lei QIU
1
;
Bao-yu GUO
;
Hong MIAO
;
Shu-yan DAO
;
Ran ZHANG
;
Peng-qun YUAN
;
Xu YANG
Author Information
- Publication Type:Journal Article
- MeSH: Adjuvants, Immunologic; pharmacology; Animals; Cell Separation; Female; Glutathione Transferase; pharmacology; Interferon-alpha; secretion; Interferon-gamma; secretion; Lymphocytes; secretion; Macrophages; drug effects; secretion; Macrophages, Peritoneal; secretion; Mice; Mice, Inbred BALB C; Protein Precursors; pharmacology; Recombinant Fusion Proteins; pharmacology; Spleen; cytology; Thymosin; analogs & derivatives; pharmacology; Tumor Necrosis Factor-alpha; secretion
- From: Acta Pharmaceutica Sinica 2002;37(5):326-328
- CountryChina
- Language:Chinese
-
Abstract:
AIMTo study the effect of prothymosin alpha (Pro T alpha) as a fusion protein on secretion of IFN-gamma, IFN-alpha and TNF-alpha in vitro.
METHODSThe in vitro study was carried out on the culture of splenocytes, splenic and peritoneal macrophages isolated from Balb/c mice. Splenocytes were incubated with various concentrations of Pro T alpha (1 x 10(-7)-1 x 10(-10) mol.L-1) with or without Con A (5 micrograms.mL-1) for 72 h. Splenic and peritoneal macrophages were respectively treated with Pro T alpha (1 x 10(-7)-1 x 10(-10) mol.L-1) in the presence of LPS (10 micrograms.mL-1) for 24 h. Then IFN-gamma, IFN-alpha and TNF-alpha levels in the supernatant were detected by ELISA.
RESULTSPro T alpha (1 x 10(-7) mol.L-1) was found to obviously increase IFN-gamma level (P < 0.05) in the supernatant of splenocytes compared with the control group. Moreover, Pro T alpha (1 x 10(-7) mol.L-1) significantly induced the secretion of IFN-alpha (P < 0.01) and TNF-alpha (P < 0.01) in splenic and peritoneal macrophages.
CONCLUSIONIn vitro, Pro T alpha could increase the secretion of IFN-gamma, IFN-alpha and TNF-alpha.
