Protective effect of tripchlorolide on dopaminergic neurons in partially lesioned rat model of Parkinson's disease.
- Author:
Xiao-xin CHENG
1
;
Feng-qiao LI
;
Min HUANG
;
Xiao-min WANG
;
Ji-sheng HAN
Author Information
- Publication Type:Journal Article
- MeSH: Animals; Behavior, Animal; drug effects; Brain; metabolism; Corpus Striatum; metabolism; Diterpenes; pharmacology; Dopamine; metabolism; Drugs, Chinese Herbal; pharmacology; Interleukin-2; metabolism; Male; Neurons; drug effects; metabolism; Neuroprotective Agents; pharmacology; Parkinson Disease; metabolism; physiopathology; Phenanthrenes; pharmacology; Rats; Rats, Sprague-Dawley; Tumor Necrosis Factor-alpha; metabolism
- From: Acta Pharmaceutica Sinica 2002;37(5):339-342
- CountryChina
- Language:Chinese
-
Abstract:
AIMTo study whether the immunosuppressant tripchlorolide (T4) exerts neuroprotective effect on dopaminergic neurons.
METHODSA rat model of Parkinson's disease (PD) was set up by transection of the medial forebrain bundle (MFB) with a wire knife. The rotational behavior, HPLC-ECD, tyrosine hydroxylase (TH) immunocytochemistry, ELISA methods were used to evaluate the influence on the dopaminergic neurons following T4 treatment.
RESULTST4 treatment was shown to effectively attenuate the rotational behavior challenged by amphetamine (2.5 mg.kg-1, i.p.) in the PD rats. T4 markedly prevented the decrease of dopamine content in the striatum and the loss of dopaminergic neurons in the substantia nigra pars compacta. T4 was found to suppress the abnormal increase of TNF-alpha and IL-2 level in brain tissues of PD rats after MFB transection.
CONCLUSIONThe evidence that the immunosuppressive Chinese herb extract T4 possesses neuroprotective activities on the dopaminergic neurons in PD rats was presented. The underlying mechanism of T4 may be relevant to its immunosuppressive activity.
