The effect and mechanism of Yiqi Tongluo Jiedu capsule against cerebral ischemia reperfusion injury.
- Author:
Yu-Yan ZHANG
1
;
Hai-Tong WAN
;
Li-Li LAI
;
Jie-Hong YANG
;
Wei-Yan CHEN
;
Hui-Fen ZHOU
;
Zhen-Hong ZHU
Author Information
1. Zhejiang Chinese Medical University, Hangzhou 310053, China.
- Publication Type:Journal Article
- MeSH:
Animals;
Brain;
metabolism;
pathology;
Capsules;
Drugs, Chinese Herbal;
administration & dosage;
isolation & purification;
pharmacology;
Infarction, Middle Cerebral Artery;
pathology;
Interleukin-1beta;
blood;
genetics;
Interleukin-6;
blood;
Male;
Malondialdehyde;
metabolism;
Nitric Oxide;
metabolism;
Nitric Oxide Synthase;
metabolism;
Plants, Medicinal;
chemistry;
Proto-Oncogene Proteins c-bcl-2;
metabolism;
RNA, Messenger;
metabolism;
Random Allocation;
Rats;
Rats, Sprague-Dawley;
Reperfusion Injury;
blood;
metabolism;
pathology;
Superoxide Dismutase;
metabolism;
Tumor Necrosis Factor-alpha;
blood;
bcl-2-Associated X Protein;
metabolism
- From:
Acta Pharmaceutica Sinica
2012;47(9):1153-1158
- CountryChina
- Language:Chinese
-
Abstract:
To observe the effect and mechanism of Yiqi Tongluo Jiedu capsule aganist cerebral ischemia reperfusion injury, the SD rats were randomly divided into following groups: sham-operated group, model group, the group of low, medium and high dose of Yiqi Tongluo Jiedu capsule, and nimodipine group. Using focal middle cerebral artery embolization (MCAO) model, following items were observed: symptoms of neurological deficit score; infarct volume; activity of SOD, content of MDA and NO, activity of NOS of ischemic brain tissue; Bcl-2 and Bax protein expression; content of IL-1beta, IL-6 and TNFalpha in serum; IL-1beta mRNA expression of ischemic brain tissue. Results showed that Yiqi Tongluo Jiedu capsule could significantly reduce the symptoms of neurological deficits, promote the recovery symptoms of neurological deficits; narrow infarct volume of brain tissue obviously, reduce the percentage of infarct volume; raise activity of SOD, reduce content of MDA and NO, reduce activity of NOS; increase Bcl-2 protein, reduce Bax expression; reduce content of IL-1beta, IL-6 and TNFa in serum; reduce IL-1beta mRNA expression of ischemic brain tissue. Yiqi Tongluo Jiedu capsule has significant protective effects against ischemic brain injury, it has significant anti-apoptotic, antioxidant and anti-inflammatory effects.
