Establishment of drug screening assay and pharmacodynamic evaluation method targeting influenza RNA polymerase.
- Author:
Zhen WANG
1
;
Xin WANG
;
Zhen HE
;
Zhen-Long LIU
;
Xiao-Lu WEI
;
Xiao YIN
;
Jin-Ming ZHOU
;
Xiao-Yu LI
;
Zhi-Zhen ZHANG
;
Shan CEN
Author Information
1. Department of lmmunology, Institute of Medicinal Biotechnology, Chinese Academy of Medical Sciences & Peking Union Medical College, Beijing 100050, China.
- Publication Type:Journal Article
- MeSH:
Amantadine;
pharmacology;
Antiviral Agents;
isolation & purification;
pharmacology;
Drug Evaluation, Preclinical;
methods;
Genes, Reporter;
HEK293 Cells;
Humans;
Influenzavirus A;
enzymology;
Luciferases;
genetics;
metabolism;
Oseltamivir;
pharmacology;
Plasmids;
RNA Replicase;
antagonists & inhibitors;
metabolism;
Reproducibility of Results;
Ribavirin;
pharmacology;
Sensitivity and Specificity;
Transfection;
Zanamivir;
pharmacology
- From:
Acta Pharmaceutica Sinica
2012;47(9):1159-1163
- CountryChina
- Language:Chinese
-
Abstract:
Influenza virus RNA-dependent RNA polymerase (RdRP) is essential for replication and expression of influenza virus genome. Viral genomic sequences encoding RdRP are highly conservative, thus making it a potential anti-influenza drug target. A cell-based influenza RdRP inhibitor screening assay was established by a luciferase reporter system to analyze the activity of RdRP. Specificity study and statistic analysis showed that the screening assay is sensitive and reproducible.
