Characterization of human anti-BAFF scFv-Fc that inhibits the activity of BAFF in vivo.
- Author:
Meng CAO
1
;
Peng CAO
;
Shuang-quan ZHANG
;
Ying CHENG
;
Huai-jiang YAN
Author Information
1. Department of Microbiology and Immunology, Medical School of Southeast University, Nanjing,China. mcao1979@yahoo.com.cn
- Publication Type:Journal Article
- MeSH:
Animals;
B-Cell Activating Factor;
immunology;
metabolism;
B-Lymphocytes;
cytology;
Body Weight;
Cell Differentiation;
Cell Proliferation;
Cells, Cultured;
Female;
Humans;
Immunoglobulin Fc Fragments;
immunology;
metabolism;
Immunoglobulin G;
blood;
immunology;
Immunoglobulin M;
blood;
Mice;
Mice, Inbred ICR;
Random Allocation;
Recombinant Fusion Proteins;
immunology;
metabolism;
Single-Chain Antibodies;
immunology;
metabolism;
Spleen;
cytology
- From:
Acta Pharmaceutica Sinica
2012;47(10):1336-1340
- CountryChina
- Language:Chinese
-
Abstract:
To investigate the effects of human anti-BAFF scFv-Fc against the hsBAFF, ICR mice were randomly divided into six groups: control, hsBAFF (1 mg x kg(-1)), hsBAFF (1 mg x kg(-1)) + Ab (1 mg x kg(-1)), hsBAFF (1 mg x kg(-1)) + Ab (2 mg x kg(-1)), hsBAFF (1 mg x kg(-1)) + human IgG (1 mg x kg(-1)) and hsBAFF (1 mg x kg(-1)) + human IgG (2 mg x kg(-1)) groups. The effects of scFv-Fc administration on the proliferation of B lymphocytes were evaluated using an MTT assay. The titres of antibody in the serum and B lymphocytes differentiation were assessed by ELISA and flow cytometry, respectively. The results showed that administration of scFv-Fc to mice injected with hsBAFF significantly prevented human BAFF-induced increases in splenic B cell numbers and serum immunoglobulin levels. Furthermore, this fully human antibody would avoid inducing the human anti-mouse antibody (HAMA) response when used in humans. These findings suggest that the compact antibody may be useful in therapeutic or diagnostic application of the BAFF-associated autoimmune diseases in human.
