Antibacterial activity and mechanism of baicalein.
- Author:
Bao-Yi YUN
1
;
Lei ZHOU
;
Kun-Peng XIE
;
Ye-Ju WANG
;
Ming-Jie XIE
Author Information
1. College of Life Science, Liaoning Normal University, Key Laboratory of Biotechnology and Drug of Liaoning Province, Dalian 116029, China.
- Publication Type:Journal Article
- MeSH:
Anti-Bacterial Agents;
isolation & purification;
pharmacology;
Bacterial Proteins;
metabolism;
Cell Membrane Permeability;
drug effects;
DNA Topoisomerases, Type I;
metabolism;
DNA Topoisomerases, Type II;
metabolism;
Flavanones;
isolation & purification;
pharmacology;
Malate Dehydrogenase;
metabolism;
Plant Roots;
chemistry;
Plants, Medicinal;
chemistry;
Scutellaria baicalensis;
chemistry;
Solubility;
Staphylococcus aureus;
cytology;
drug effects;
metabolism;
Succinate Dehydrogenase;
metabolism
- From:
Acta Pharmaceutica Sinica
2012;47(12):1587-1592
- CountryChina
- Language:Chinese
-
Abstract:
Baicalein (BAI) is an effective bactericide. The antibacterial activity and mechanism experiments were carried out by determining conductivity and content of macromolecules of membrane penetrability, the oxidative respiratory metabolism and protein synthesis changes and the inhibition of DNA topoisomerase activities. Electrical conductivity and the number of large molecules of BAI increased 2.48% and 1.8%, respectively, than that of the control. However, the membrane integrity did not destroyed by BAI directly. With BAI treatment, inhibition rates of activities for SDH and MDH were 56.2% and 57.4%, respectively, demonstrating that BAI could inhibit cell respiratory. After treated with BAI for 20 h, the total soluble content of proteins decreased by 42.83%. Moreover, the activities of DNA topoisomerase I and II were inhibited completely by 0.2 mmol x L(-1) BAI. These results indicated that BAI had obvious antibacterial activity on Staphylococcus aureus. The mechanism is that it could affect bacterial membrane penetrability, inhibit protein synthesis and influence SDH, MDH and DNA topoisomerase I and II activities to exert its antibacterial functions.
