Photo-activated DNA binding and antimicrobial activities of alkaloids from Glycosmis pentaphylla.
- Author:
Yu CHEN
1
;
Bo YANG
;
Jing XU
;
Tong ZHENG
;
Hua FAN
;
Guang-Zhong YANG
Author Information
1. College of Chemistry and Material Sciences, South Central University for Nationalities, Wuhan 430074, China.
- Publication Type:Journal Article
- MeSH:
Anti-Bacterial Agents;
chemistry;
isolation & purification;
pharmacology;
Bacillus subtilis;
drug effects;
Carbazoles;
chemistry;
isolation & purification;
pharmacology;
Chromatography, Thin Layer;
Coumaric Acids;
chemistry;
isolation & purification;
pharmacology;
DNA Fragmentation;
DNA Restriction Enzymes;
metabolism;
Light;
Molecular Structure;
Photosensitizing Agents;
pharmacology;
Plants, Medicinal;
chemistry;
Protein Binding;
Rutaceae;
chemistry;
Staphylococcus aureus;
drug effects;
Ultraviolet Rays
- From:
Acta Pharmaceutica Sinica
2012;47(12):1646-1652
- CountryChina
- Language:English
-
Abstract:
In our screening for photosensitizers from natural resources, four alkaloids were isolated from Glycosmis pentaphylla by various chromatography techniques. Their structures were identified as glycoborinine (1), glybomine B (2), carbalexin A (3) and N-p-coumaroyltyramine (4) by spectral analysis. Their photoactivated antimicrobial activities were evaluated by thin-layer chromatography (TLC) agar overlay assay against Staphylococcus aureus and Bacillus subtilis. It was found that compounds 1 and 4 showed photo-activated antimicrobial activities. Meantime, photo-activated DNA binding activities of these compounds were also assessed by using a specially prepared 1.8 kb DNA fragment and restriction enzymes. Under UVA irradiation, compound 1 showed moderate inhibition on Nde I, Xba I, Nco I and Bcl I which have either 5'-TpA or 5'-ApT and trace or no inhibition on other restriction enzymes. It showed a similar inhibition pattern with the reference 8-methoxypsoralen. However, compounds 2-4 showed no inhibition against any of the restriction enzymes.
