Determination of doping in human urine by gas chromatography-high resolution mass spectrometry.
- Author:
Yan-Yi XING
1
;
Xin LIU
;
Yu-Mei ZHANG
;
Xiao-Bing WANG
;
You-Xuan XU
Author Information
1. China Anti-Doping Agency, Beijing 100029, China. xingyanyi@tom.com
- Publication Type:Journal Article
- MeSH:
Anabolic Agents;
urine;
Doping in Sports;
Gas Chromatography-Mass Spectrometry;
Humans;
Methandrostenolone;
urine;
Nandrolone;
urine;
Substance Abuse Detection;
methods;
Testosterone;
analogs & derivatives;
urine
- From:
Acta Pharmaceutica Sinica
2012;47(12):1667-1670
- CountryChina
- Language:Chinese
-
Abstract:
A method was evaluated for determination of twenty-one doping (including nandrolone, boldenone and methandienone) in human urine by gas chromatography-high resolution mass spectrometry. Samples were prepared by liquid-liquid extraction, concentrated, TMS derivatization and limit of detection at ng x mL(-1) by MID/GC/HRMS. According to the code of the World Anti-Doping Agency (WADA), precision and recoveries of the procedure were evaluated by replicate analysis (n = 6), the recoveries in the range of 66%-103%, with the RSD below 10.0%. The precision within the day of the method with three different concentrations was also determined RSD were less than 9.5%, 10.0% and 9.7%.
