The p53-p21waf1 pathway and centrosome amplification in oral squamous cell carcinomas
10.3760/cma.j.issn.1002-0098.2009.06.004
- VernacularTitle:p53-p21waf1通路在口腔鳞状细胞癌中心体扩增中的作用及意义
- Author:
Yang CAI
1
;
Yong-Fa LIU
;
Hong YANG
;
Hong LU
Author Information
1. 贵阳医学院
- Keywords:
Carcinoma,squamous cell;
Centrosome;
Flow cytometry;
p21waf1
- From:
Chinese Journal of Stomatology
2009;44(6):332-335
- CountryChina
- Language:Chinese
-
Abstract:
Objective To elucidate the possible role of p53-p21waf1 pathway for centrosome amplification in oral squamous cell carcinoma ( OSCC ) . Methods Formalin-fixed, paraffin-embedded tissues of 8 cases of normal oral epithelium tissues and 27 cases of OSCC tissues were examined for the expression of p21waf1 and mutated p53 proteins by flowcytometry and immunohistochemistry, and centrosome status was investigated by indirect immunofluorescence double staining with antibodies to centrosome protein gamma-tubulin and cytokeratin. The correlation between p21waf1, p53 and centrosome amplification in OSCC was statistically analyzed by SPSS 12.0. Results All normal oral epithelium tissues showed normal centrosomes( 1-2 centrosomes per cell) in epithelium cells, while 21 out of 27 cases(78%) of OSCC showed the evidence of centrosome amplification characterized by supernumerary centrosomes (＞2 centrosomes per cell) in a fraction of tumor cells. The quantity of p21waf1 protein was lower in OSCC with centrosome amplification [(0. 878±0. 081 )] than that in OSCC without centrosome amplification [ (0. 952±0. 018 ) ,t =3.838,P<0.01] , and negative correlations were found between the quantity of p21waf1 protein and the degree of centrosome amplification ( r = - 0.472, P＜0. 05) , as well as the positive staining of p53 ( r =-0.491 ,P＜0. 01). Conclusions p53-p21waf1 pathway might involve in centrosome duplication cycle in OSCC. Down-regulated p21waf1 protein, via p53 transactivation-dependent mechanism, was likely a contributing factor towards centrosome amplification in OSCC.
