Mutation and expression of tumor suppressor gene phosphatase and tensin homolog deleted in chromosome 10 in oral squamous cell carcinoma

VernacularTitle:口腔鳞状细胞癌中蛋白酪氨酸磷酸酶基因的突变与表达
Author: Qian LI ¹ ; Ji-Zhi ZHAO ; Yong-Ning CHEN ; Tao ZHANG ; Lian ZHOU ; Ye JIN
Author Information

1. 中国医学科学院中国协和医科大学北京协和医院
Keywords: Carcinoma,squamous cell; Protein-tyrosine-phosphatase; Genes
From: Chinese Journal of Stomatology 2009;44(11):660-663
CountryChina
Language:Chinese
Abstract: Objective To investigate the role of mutation and mRNA expression of tumor suppressor gene phosphatase and tensin homolog deleted in chromosome 10(PTEN)in tumorigenesis and progression of oral squamous cell carcinoma(OSCC).Methods The mutation of exon 3,5,6 and exon 8 of PTEN gene in 42 oral squamous cell carcinoma tissue and paired normal tissue were detected by polymerase chain reaction(PCR)and DNA sequencing methods.The levels of PTEN mRNA expression in these tissues were assayed using semi-quantitative RT-PCR.Data Was analyzed with SPSS 14.0 software package.Results Mutated exon 5 of PTEN gene Was found in 2 cases of advanced OSCC.The expression of PTEN mRNA Was detected in all OSCC and paired normal tissue.The level of PTEN mRNA in OSCC tissue(0.36±0.12)Was significantly lower than that of paired normal tissue(0.64±0.09,P＜0.01).Conclusions The decreased expression of PTEN mRNA contributes to tumorigenesis of OSCC.